| 羊和猪胎盘肽的制备及其生物活性的初步研究 |
| |
| 引用本文： | 周国华,孟凡军,王永志,葛利江.羊和猪胎盘肽的制备及其生物活性的初步研究[J].家畜生态,2006(3). |
| |
| 作者姓名： | 周国华 孟凡军 王永志 葛利江 |
| |
| 作者单位： | 山东农业大学动物科技学院 山东泰安271018(周国华，葛利江)，山东省沂水县畜牧局 276400山东沂水(孟凡军)，济南市农业学校 山东济南250023(王永志) |
| |
| 摘 要： | 利用超滤法从羊和猪胎盘中提取出了胎盘肽,采用改良的加脲Tricine-SDS-PAGE电泳法测定羊胎盘肽的分子量为4,386Da;利用紫外光扫描得其最大吸收峰分别为225.4 nm和209.6 nm;并通过建立体外抑制兔淋巴细胞模型,利用MTT比色法测定所提取胎盘肽的免疫调节作用,结果显示:羊和猪胎盘肽均可使顺铂抑制的兔外周血T淋巴细胞的转化率显著提高,其中羊胎盘肽以1:102、1:103和1:104三组效果最为明显(P<0.01);而猪胎盘肽以1:102、1:103两组效果最为明显(P<0.01)。
|
| 关 键 词： | 胎盘肽 加脲Tricine-SDS-PAGE电泳 免疫抑制 MTT比色法 |
Preparation of Caprine and Swine Placental Peptide and Studies on Its Biological Activity |
| |
| ZHOU Guo-hua,MENG Fan-jun,WANG Yong-zhi,GE Li-jiang.Preparation of Caprine and Swine Placental Peptide and Studies on Its Biological Activity[J].Ecology of Domestic Animal,2006(3). |
| |
| Authors: | ZHOU Guo-hua MENG Fan-jun WANG Yong-zhi GE Li-jiang |
| |
| Institution: | ZHOU Guo-hua~1,MENG Fan-jun~2,WANG Yong-zhi~3,GE Li-jiang~ |
| |
| Abstract: | The distilled caprine and swine placental peptides were prepared by ultrafilter and the molecular weight of caprine placental peptide which was 4,386Da was mensurated by improved Tricine-SDS-PAGE eletrophoresis with urea.Their absorbance peaks which were respectively 225.4nm and 209.6nm were got by ultraviolet scanning.Their immnoregulatory effects were mensurated by MTT Colorimetric Assay after establishing a model of in vitro immunosuppressive lymphocytes of rabbit.The results in the experiment showed that the bovine and caprine placental peptides could improve immunosuppressive T lymphocyte transformation rate.The immunosuppressive lymphocyte transformation rates in groups with the caprine placental peptides of 1:10~2,1:10~3and 1:10~4(P<0.01)and with the swine placental peptides of 1:10~2,1:10~3(P<0.01)increased more remarkably than that in immunosuppression group. |
| |
| Keywords: | placental peptide Tricine-SDS-PAGE eletrophoresis with urea immunosuppression MTT Colorimetric Assay |
| 本文献已被 CNKI 等数据库收录！ |
