| 应用半嵌套PCR扩增鹿源狂犬病野毒核蛋白基因 |
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| 引用本文： | 李影,段锐,钱爱东. 应用半嵌套PCR扩增鹿源狂犬病野毒核蛋白基因[J]. 经济动物学报, 2004, 8(4): 202-204 |
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| 作者姓名： | 李影 段锐 钱爱东 |
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| 作者单位： | 吉林农业大学动物科技学院,长春,130118;辽源市畜牧兽医总站,辽源,136200 |
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| 基金项目： | 国家自然科学基金资助项目 (3 0 0 70 5 42 ) |
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| 摘 要： | 应用一对外引物 (RNP1 ,RNP2 )和一个套式引物 (RNP3) ,对鹿源狂犬病野毒 82 0 2株核蛋白基因 (约135 0bp)进行 2次PCR扩增。第 1次仅扩增出一条约 75 0bp的片段 ,第 2次扩增时 ,将第一次扩增产物进行10 3倍稀释后能较好地扩增出目的片段。结果表明 ,半套式PCR引物的非特异性对目的片段的干扰 ,具有较高的敏感性。
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| 关 键 词： | 鹿源狂犬病病毒 核蛋白 丰嵌套聚合酶链式反应 |
| 文章编号： | 1007-7448(2004)04-0202-03 |
| 修稿时间： | 2004-09-12 |
Amplification of the Nucleoprotein Gene of Deer Rabies Virus with Semi-nested PCR |
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| LI Ying,DUAN Rui,QIAN Ai-dong. Amplification of the Nucleoprotein Gene of Deer Rabies Virus with Semi-nested PCR[J]. Journal of Economic Animal, 2004, 8(4): 202-204 |
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| Authors: | LI Ying DUAN Rui QIAN Ai-dong |
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| Affiliation: | LI Ying1,DUAN Rui2,QIAN Ai-dong1 |
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| Abstract: | The research applied two primers(RNP 1,RNP 2)and a semi-nesnested primer(RNP 3)to amplify the nucleoprotein gene of rabies virus from deer. Only a fragment about 750 bp was amplified at first PCR.The ncleoprotein gene of rabies virus was amplified at second PCR after the produce from first PCR was diluted 103times.The results showed semi-nested PCR not only overcame the interference from non-special primers in normal PCR,but also improved the PCR sensitivity. |
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| Keywords: | deer rabies virus nucleoprotein semi-nested PCR |
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