Fluorescent staining of resting spores of Polymyxa betae as a fungal vector of rhizomania disease of sugar beet in soil |
| |
| Authors: | Mitsuru Sayama Yoji Momota Shigehito Takenaka |
| |
| Affiliation: | (1) Department of Upland Agriculture, National Agricultural Research Center for Hokkaido Region, Shinsei, Memuro, Hokkaido, Japan;(2) Department of Agro-Environment Sciences, National Agricultural Research Center for Hokkaido Region, Sapporo, Japan;(3) Present address: National Agricultural Research Center for Hokkaido Region, Hitsujigaoka, Sapporo 062-8555, Japan;(4) Present address: Department of Research Planning and Coordination, National Agricultural Research Center, Tsukuba, Japan |
| |
| Abstract: | When the resting spores of Polymyxa betae were pretreated with 2% sodium dodecyl sulfate (SDS) and then stained with various fluorochromes including 3,3′-dihexyloxacarbocyanine iodide [DiOC6(3)], calcofluor, and a fluorescein isothiocyanate (FITC)-conjugated lectin, such as wheat germ lectin (WGA) or caster bean lectin, most spores fluoresced brightly. FITC-WGA mainly stained the cell surface, while DiOC6(3) stained the cytoplasm. After pretreatment with SDS and addition of FITC-WGA or DiOC6(3) to a soil suspension containing resting spores, the resting spores were distinguishable from the soil particles. This staining method is easy to use for the detection of resting spores in the soil. |
| |
| Keywords: | Fluorochrome Lectin Polymyxa betae Rhizomania Sugar beet |
| 本文献已被 SpringerLink 等数据库收录！ |
|
