桑蚕蛹期卵巢移植及其生物学效应的研究

本文报导桑蚕在蛹期进行卵巢移植试验的成功,并调查了卵巢移入雄蛹后的生物学效应。明确了血液中卵黄原蛋白向卵巢移行的时期是在蛹期。试验表明:蛹龄2h的雌、雄蛹在低温抑制后进行移植为适宜;卵巢植入雄蛹后受体发育良好,羽化率达82.5％～100％;雄蛹内卵巢能形成成熟卵,但卵大小、重量和卵肉蛋白质、核酸含量以及卵超微结构发生了一些变化;用人工单性生殖方法处理雄蛹内的发育卵,得到14.2％～16.0％的成胚率,进一步研究可望获得后代。

STUDIES ON THE TRANSPLANTATION OF THE SILKWORM OVARY AT THE PUPAL STAGE AND ITS BIOLOGICAL EFFECT

This paper describes a successful transplantation of the silkworm at the pupal stage. The biological effect after the implantation of the ovary into the male pupa was investigated, and the period during which transferance of vitellogenin to the ovary via the blood occurred was identified as the pupal stage. The result of the experiment was as follows; for the 2-hr aged male and female pupa, it was most suitable to do the transplantaton after low temperature treatment of the pupae; after the ovary was implanted into the male pupa, the rcipient developed very well, the rate of emergence reaching 82. 5 % - 1 00%, Mature eggs formed inside the ovary of the male pupa, though there were some changes in size, weight, protein content, nuclec acid content and ultrastructure of the eggs. After parthenogenesis treatment, 14. 2% -16. 8% of the eggs inside the male pupa developed into early embryos and it should be possible to have progenies if further study is conducted.