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| 球虫抗药性分子生物学检测技术的建立 | |
| 引用本文： | 安健,汪明,王黎霞,盛守强. 球虫抗药性分子生物学检测技术的建立[J]. 中国兽医学报, 2007, 27(3): 340-342,346 |
| 作者姓名： | 安健 汪明 王黎霞 盛守强 |
| 作者单位： | 1. 北京农学院,动物科学技术系,北京,102206 2. 中国农业大学,动物医学院,北京,100094 3. 河北省张家口市康保县康保一中,河北,康保,076650 |
| 基金项目： | 国家科技攻关项目;北京市自然科学基金 |
| 摘 要： | 选择在敏感虫株中沉默、抗药虫株中表达的特异序列AGD5设计检测引物，采用RT—PCR方法。以总RNA反转录的cDNA为模板，RT—PCR方法能够鉴别马杜霉素抗药虫株和3株马杜霉素敏感虫株（2株对所有药物敏感的不同地理株，1株地克珠利抗药性虫株对马杜霉素敏感），此鉴定结果与鸡体试验的结果一致，说明敏感虫株和抗药虫株在该序列上的差异发生在转录水平。而以卵囊总DNA为模板的PCR方法不能鉴别敏感和马杜霉素抗药性虫株。 |
| 关 键 词： | 柔嫩艾美耳球虫 抗药性 检测 |
| 文章编号： | 1005-4545（2007）03-0340-03 |
| 修稿时间： | 2005-09-10 |
Identification of the drug resistant strain by molecular biological technique |
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| An Jian,Wang Ming,WANG Li-xia,SHENG Shou-qiang. Identification of the drug resistant strain by molecular biological technique[J]. Chinese Journal of Veterinary Science, 2007, 27(3): 340-342,346 | |
| Authors: | An Jian Wang Ming WANG Li-xia SHENG Shou-qiang |
| Affiliation: | 1. Department of Animal Science and Technology, Beijing Agricultural College, Beijing 102206, China ; 2. College of Veterinary Medicine China Agricultural University, Beijing 100094, China; 3. Kangbao No. 1 middle school,Kangbao, Hebei 076650, China |
| Abstract: | The sense and anti-sense primers were designed according the special sequence from the resistant strain by the result of the differential display PCR. The total RNA was purified with Trizol from merozoite of 1 maduramisin resistant E. tenella strain and 3 differntial maduramisin sensitive E. tenella strains. The DNA was purified with DNA purification kit from oocysts of the 4 differential strains. The drug resistance could be identified by RT-PCR. It was unanimous to the test results in chickens. But it could not be identified by PCR with DNA template. |
| Keywords: | PCR RT-PCR |
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