| 草莓品种亲缘关系的AFLP分子标记分析 |
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| 引用本文： | 张运涛,冯志广,李天忠,董静,王桂霞,张开春,韩振海. 草莓品种亲缘关系的AFLP分子标记分析[J]. 园艺学报, 2006, 33(6): 1199-1202. DOI: 1000-4718 |
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| 作者姓名： | 张运涛 冯志广 李天忠 董静 王桂霞 张开春 韩振海 |
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| 作者单位： | (北京市农林科学院林业果树研究所, 北京100093; 中国农业大学园艺植物研究所, 北京100094) |
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| 基金项目： | 北京市科委资助项目(H012010390113) |
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| 摘 要： | 利用AFLP技术对我国国家草莓资源圃内保存的107个草莓品种进行了DNA多态性分析。从64对“2 + 2”引物中筛选出10对引物应用于扩增基因组DNA, 共获得清晰可辨的581条带, 其中412条为多态性带。UPGMA方法聚类分析结果显示, AFLP分子鉴定结果与其系谱关系非常一致, 表明AFLP指纹图谱分析能很好地鉴别草莓品种之间的遗传关系。
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| 关 键 词： | 草莓 AFLP 亲缘关系 分子鉴定 |
| 文章编号： | 0513-353X（2006）06-1199-04 |
| 收稿时间： | 2006-04-28 |
| 修稿时间： | 2006-04-282006-09-13 |
Genetic Relationships of Strawberry Cultivars by AFLP Analysis |
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| Zhang Yuntao,Feng Zhiguang,Li Tianzhong,Dong Jing,Wang Guixia,Zhang Kaichun,Han Zhenhai. Genetic Relationships of Strawberry Cultivars by AFLP Analysis[J]. Acta Horticulturae Sinica, 2006, 33(6): 1199-1202. DOI: 1000-4718 |
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| Authors: | Zhang Yuntao Feng Zhiguang Li Tianzhong Dong Jing Wang Guixia Zhang Kaichun Han Zhenhai |
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| Affiliation: | 1.Department of Biochemistry,Guangdong Medical College,2Guan gdong Key Laboratory for Research and Development of Natural Drugs,Zhanjiang 52 4023,China;3.Shanghai Institute of Hematology,Shanghai Second M edical School affiliated Ruijin Hospital,Shanghai 200025,China |
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| Abstract: | The AFLP analysis was conducted in order to investigate the blood relationship among the 107 strawberry cultivars conserved in Beijing,the National Strawberry Germplasm Repository.10 pairs primers were selected from 64 primer pairs(2 2)to amplify the genomic DNAs.Total 581 AFLP fragments were obtained,412 of them were polymorphic markers.The phylogenetic tree generated by UPGMA method showed the genetic relationship among cultivars identified by AFLP was better identical with their pedigree.AFLP technique was proved to be a very reliable method to study the genetic relationships of strawberry cultivars. |
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| Keywords: | Strawberry AFLP Genetic relationship Molecular identification |
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