中国亚热带常见园林植物清除DPPH自由基活性研究

 采用二苯基苦基苯肼(DPPH) 自由基酶标仪法, 对中国亚热带常见的83 科164 种园林绿化植物鲜叶的自由基清除活性进行了分析比较, 结果表明, 清除活性强(清除50 % DPPH 自由基时的样品浓度IC₅₀ < 0.500 mg·mL ^-1) 的有25 种, 清除活性中等( IC₅₀ 0.501～1.000 mg·mL ^-1) 的31 种, 清除活性弱( IC₅₀> 1.001 mg·mL ^-1 ) 的108 种。其中石榴、金缕梅、枫香、三角枫、木、算盘子、山杜英、青冈栎、棣棠、南天竹等10 种植物鲜叶DPPH 自由基清除活性IC₅₀ < 0.400 mg·mL^-1, 其它种IC₅₀值介于0.401～14.300mg·mL^-1之间。讨论了其作为天然自由基清除剂或天然抗氧化剂产品新资源开发利用的前景。

Studies on Scavenging Activity Against DPPH Free Radical in Fresh Leaves from Some Common Subtropical Plants of Garden and Afforestion in China

A DPPH-Microplate assay was made to determine the free radical scavenging activity in leaves of subtropical plants of garden and afforestion belonging to 83 Families 164 Species. The results showed it can be divided into three types of high (sample concentration of scavenging 50 percent of DPPH free radical IC₅₀ < 0.500 mg ·mL ^-1) , medium ( IC₅₀0.501 - 1.000 mg·mL ^-1 ) and low ( IC₅₀ > 1.001 mg·mL ^-1) free radical scavenging activity. There are 25 with high activity , 31 with medium, 108 with low. Among them, Pomegranate, Chinese Witchhazel, Beautiful Sweetgum, Buerger Maple, Chinese Loropetalum, Puberulous Glochidion , Sylvestral E2-aeocarpus , Blue Japanese Oak , Japanese Kerria and Common Nandina have the highest activity.Their IC₅₀ are 0.283、0.292、0.312、0.326、0.345、0.369、0.379、0.389、0.398、0.403 mgmL^-1,respectively.Others IC₅₀ is between 0.401～14.300 mg·mL ^-1 . It is suggested that these species can be used a new resource of free radical scavenger and antioxidant products.