盐胁迫下枣叶片蛋白质组差异的分析

【目的】为了探明盐胁迫下枣蛋白表达的差异,【方法】以较耐盐碱的‘七月鲜’枣品种的扦插苗为材料,经过临界浓度(0.60%)的钠盐(NaCl)处理,取其叶片进行蛋白质双向电泳,经ImageMaster 2D分析,【结果】结果表明,处理与对照之间总蛋白的整体分布模式非常相似,在pH 3~10和MW50~90 kD内的蛋白点分布最多,处理与对照之间量变倍数大于1.5倍的差异点有26个,其中6个蛋白受胁迫上调,20个蛋白受胁迫下调。在上调的蛋白质点中,3个点受盐胁迫诱导增加了近4倍,2个蛋白质点相对丰度增加了2倍以上,表现出强烈的盐诱导特性。说明他们可能在枣的抗盐机制中发挥重要作用。在下调的蛋白质点中,2个点相对丰度降低至对照的5倍左右,表现出强烈的盐抑制特性,说明其可能是对盐胁迫比较敏感的2个蛋白质或多肽,并且在枣的抗盐性机制中也发挥比较重要的作用。【结论】在临界浓度钠盐胁迫下,枣叶片蛋白质表达有显著差异。

Studies on the proteomic difference of Chinese jujube(Ziziphus jujuba) leaf under salt stress

【Objective】The objective of this paper is to prove the protein expression difference of Chinese jujube(Ziziphus jujuba Mill.)under salt stress.【Method】The cutting-propagated sprouts of saline-alkali tolerant cultivar 'Qiyuexian' were selected as experiment materials and treated by over critical concentration(0.60%) of NaCl,then sampled leaves for two-dimensional electrophoresis of proteins.【Result】After ImageMaster 2D analysis,the result was as follows: The whole proteins' integral distribution pattern was very similar between treatment and CK,the most protein spots were scattered among pH 3-10 and MW50-90kD.The relative abundance of twenty-six spots varied more than one and a half times between treatment and CK,six of them were up-regulated by salt stress,and the rest were down-regulation.In the up-regulation protein spots,three of their quantity change was near four times compared with that of CK,while two of them raised 2 times,which suggested those proteins might play an important role in the mechanism of salt-tolerance.Among the down-regulation protein spots,2 543 and 2 498 were restrained maximumly by salt-intimidation,their relative abundance decreased five times than that of contrast,the protein expression was strongly inhibited by salt stress,which suggested those two proteins or polypeptides might be susceptible to salt-intimidation and also played an important role in the mechanism of salt-tolerance.The other protein spots which relative abundance reduced greatly by the restraint of salt-intimidation were 1 527,287,647,492.【Conclusion】The sodium salt stress in the critical concentration could significantly change the protein expression of dates leaves.