Determination of S-RNase genotypes and isolation of four novel S-RNase genes in Japanese apricot (Prunus mume Sieb. et Zucc.) native to China |
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| Authors: | P P Wang T Shi W B Zhuang Z Zhang Z H Gao |
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| Institution: | 1. College of Horticulture, Nanjing Agricultural University, No. 1 Weigang, Nanjing City 210095, Jiangsu Province, P. R. Chinagaozhihong@njau.edu.cn;3. College of Horticulture, Nanjing Agricultural University, No. 1 Weigang, Nanjing City 210095, Jiangsu Province, P. R. China |
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| Abstract: | SummaryJapanese apricot (Prunus mume Sieb. et Zucc.) originated in China with more than 500 cultivars. Most cultivars are self-incompatible. It is essential to identify S-genotypes for production and breeding. The S-genotypes of 17 Japanese apricot cultivars native to China were determined using the Prunus S-RNase consensus primer pair, Pru-C2 and PCE-R, which were designed from the second and third conserved regions of Prunus S-RNases, respectively. Eleven S-RNase alleles (S1 S2, S7, S12, S14, S15, S18, S20, S22, S23 and S26) and four new S-RNase alleles (S30, S31, S32 and S33) with GenBank Accession Numbers JN232975, JN232976, JN232977, JN232978 were identified. Furthermore, the S-genotypes of four Japanese apricot cultivars were confirmed by field-testing for cross-pollination. The results of this study enrich the information available on S-genotypes in Japanese apricot and provide a reasonable basis for the appropriate arrangement of pollination trees in orchard practice. |
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