Meristem culture and micropropagation of a variety of ginger (Zingiber officinale Rosc.) with a high yield of oleoresin |
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Authors: | B. Bhagyalakshmi Narendra S. Singh |
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Affiliation: | 1. Department of Applied Botany, University of Mysore, Mysore 570 006, India;2. Area of Fruits, Vegetables and Plantation Crops, Central Food Technological Research Institute, Mysore 570 013, India |
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Abstract: | SummaryMeristems of ginger with or without leaf primordia were induced to form shoots on three-quarter strength Murashige-Skoog’s (MS) medium containing sucrose 6%, coconut milk (CM) 20%, ascorbic acid (AA) 100 mg l?1, glutamine (GL) 400 mg l?1, activated charcoal (AC) 250 mg l?1, 6-benzylaminopurine (BAP) 0.5 mg l?1, indolebutyric acid (IBA) 0.4 mg l?1 and agar 0.8%. Meristem-derived shoots exhibited consistent multiplication on three-quarter strength MS medium containing sucrose (3%), AA (100 mg l?1), AC (100 mg l?1), BAP (4–5 mg l?1) and agar (0.8%). Liquid media (agitated or static) were less effective than a solid (agar-gelled) medium for micropropagation. Kinetin and naphthalene acetic acid (NAA) incorporated at various levels (0.01–0.8 mg l?1) with or without added BAP and IBA neither improved plantlet formation nor enhanced shoot multiplication. The in vitro plants were successfully established in vivo and the rhizome yield was comparable with that of plants grown by conventional methods. |
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