Comparison of cell membrane thermostability and chlorophyll fluorescence parameters for the determination of heat tolerance in ten cabbage lines

Summary

Cell membrane thermostability (CMT) and chlorophyll fluorescence (CF) were determined for ten lines of cabbage in order to select heat-tolerant varieties, to provide an improved method to screen for heat tolerance, and to determine whether there is a relationship between CMT and CF. Chlorophyll fluorescence parameters, especially variable fluorescence (Fv) and the ratio between Fv and maximum fluorescence (Fm), were found to be better than CMT for screening cabbage lines for heat tolerance. Fv values and the Fv/Fm ratio of stressed plants corresponded to high heat damage for the varieties ‘HRI 002605’, ‘HRI 003202’, and ‘HRI 007827’; and to low heat damage for the varieties ‘HRI 013011’, ‘HRI 005237’, and ‘HRI 006556’. The latter group may therefore be more tolerant of high temperature stress in the tropics. There were significant relationships (P < 0.05) between relative injury (RI), an index of CMT, and two of the CF parameters miniumum florescence (Fo) and Fv/Fm] under stress conditions (35° – 40°C). This suggests that parameters measured under stress temperatures are more reliable than those measured under non-stressed conditions when determining heat tolerance.