Development of new microsatellite markers for molecular diversity analysis of Citrus species |
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| Authors: | A Dixit JW Chung WG Zhao GA Lee DH Lee KH Ma |
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| Institution: | 1. Department of Plant Resources, College of Industrial Science, Kongju National University, Yesan 340-702, Republic of Korea;2. Rice DNA and Quality Testing Laboratory, Basmati Export Development Foundation, SVBP University of Agriculture and Technology, Meerut 250110, India;3. Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Jiangsu University of Science and Technology, Zhenjiang 212018, P. R. China;4. National Agrobiodiversity Center, National Institute of Agricultural Biotechnology, RDA, Suwon, 441-707, Republic of Korea;5. Citrus Research Station, National Institute of Horticultural &6. Herbal Science, RDA, Suwon 369-873, Republic of Korea |
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| Abstract: | SummaryThirty microsatellite loci for further genetic analysis of Citrus species were developed by constructing a microsatellite-enriched library using capture with streptavidin-coated magnetic beads and used to assess genetic diversity in 40 Citrus accessions. In total, 150 alleles were detected, with an average of five alleles per locus. The average gene diversity and polymorphism information content values were 0.58 and 0.52, and ranged from 0.35 – 0.74 and from 0.32 – 0.70, respectively. Values for the observed and expected heterozygosities ranged from 0.13 – 1.00 and from 0.36 – 0.75, respectively. Fifteen loci deviated from the Hardy-Weinberg equilibrium (P < 0.05). The mean similarity coefficient among accessions was 0.5906. Based on the UPGMA algorithm, two main groups were successfully identified. These new microsatellite markers can be used to further investigate the genetics of, and phylogenic relationships in Citrus spp. |
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