Diversity of S-RNase genes and S-haplotypes in Japanese plum (Prunus salicina Lindl.) |
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Authors: | Kenji Beppu Hisayo Yamane Hideaki Yaegaki Masami Yamaguchi Ikuo Kataoka Ryutaro Tao |
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Institution: | 1. Faculty of Agriculture, Kagawa University, Miki, Kagawa 761-0795, Japanbeppuk@ag.kagawa-u.ac.jp;3. Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan;4. National Institute of Fruit Tree Science, Tsukuba, Ibaraki 305-8605, Japan;5. Faculty of Agriculture, Kagawa University, Miki, Kagawa 761-0795, Japan |
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Abstract: | SummaryThis report demonstrates the diversity of S-haplotypes in Japanese plum by molecular cloning of genomic DNAs and cDNAs that encode S-RNases. Nine different DNA fragments, designated as Sa–Si, were obtained from 17 Japanese plum cultivars by PCR with an S-RNase gene-specific primer set, Pru-C2 and PCE-R. Eleven different S-haplotypes were found in these cultivars. The banding patterns obtained with another S-RNase gene-specific primer set, Pru-T2 and PCE-R, corresponded to the S-haplotypes predicted from the Pru-C2 and PCE-R primer set. Several cultivars had the same S-haplotypes. Partial genomic DNAs for eight S-RNase genes and cDNAs for two S-RNases were cloned and sequenced. Deduced amino acid sequences contained conserved regions among the rosaceous S-RNases. Comparisons of the sequences from cDNAs and genomic DNAs revealed the presence of two introns in the S-RNase genes of Japanese plum as in other Prunus S-RNase genes. Pollination incompatibility groups and self-compatibility in Japanese plum were discussed with reference to the S-haplotypes. |
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