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A novel method for Citrus propagation: Seed grafting
Authors:Moshe Bar-Joseph  Cecile Robertson  E Hilf Mark  O Dawson William
Institution:1. GimlaoTec, Hazanchanim 8, Rehovot, 76211 Israelmbjoseph@gmail.com;3. Citrus Research and Education Center, University of Florida-IFAS, Lake Alfred, FL 33850, USA;4. US Horticultural Research Laboratory, Fort Pierce, Florida, 34945, USA
Abstract:Summary

We have developed a novel technique for grafting citrus seeds onto citrus rootstock plants that resulted in successful graft-take with normal vascular connections between the emerging seedling stem tissues and the rootstock plant. The method was found to be suitable for producing grafted plants from seeds of six cultivars and hybrids of Citrus and the citrus relative Murraya paniculata, using four common Citrus rootstocks. Plants produced by this method developed normally and were established in the field more rapidly than those produced by the common practice of grafting the rootstocks with budwood derived from seedlings prepared from seed in soil-based media. Seed grafting is expected to find a range of uses in breeding programmes; for example, by reducing the time required for the evaluation of hybrid seedlings, in cases where the female parent is mono-embryonic, for testing for vertical transmission of pathogens, and for screening for pathogen resistance among hybrid and mutagenised seed sources.
Keywords:
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