| 野生赭鳞蘑菇及其分离菌株的鉴定 |
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| 引用本文： | 宋小亚,刘德云,李阳,顾新伟,吴春玲.野生赭鳞蘑菇及其分离菌株的鉴定[J].食用菌学报,2012,19(1):27-35. |
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| 作者姓名： | 宋小亚 刘德云 李阳 顾新伟 吴春玲 |
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| 作者单位： | 1. 丽水市农业科学研究院食用菌研究所,浙江丽水,323000
2. 丽水市农业局,浙江丽水,323000 |
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| 基金项目： | 浙江省重大科技专项重点农业项目，浙江省食用菌产业创新团队项目 |
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| 摘 要： | 依据形态学特征结合rDNA-ITS分析,对采集到的野生真菌进行分类鉴定;采用RAPD分析生物学手段快速判断其分离菌株的真实性,并通过出菇试验验证判断结果。该野生真菌鉴定为赭鳞蘑菇(Agaricus subrufescens),RAPD分析和出菇试验结果表明分离菌株为该野生真菌的纯培养物。
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| 关 键 词： | 野生真菌 赭鳞蘑菇 分离菌株 鉴定 |
Identification of an Agaricus subrufescens Strain from Zhejiang Province, China |
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| SONG Xiaoya
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LIU Deyun
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LI Yang
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GU Xinwei
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WU Chunling.Identification of an Agaricus subrufescens Strain from Zhejiang Province, China[J].Acta Edulis Fungi,2012,19(1):27-35. |
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| Authors: | SONG Xiaoya LIU Deyun LI Yang GU Xinwei WU Chunling |
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| Institution: | 1(1Edible Fungi Institute,Lishui Agricultural Academy of Sciences,Lishui,Zhejiang 323000,China;2Lishui Agricultural Bureau,Lishui,Zhejiang 323000,China) |
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| Abstract: | Mushroom fruit bodies,collected from bamboo leaf piles near Bihu Town,Lishui City,Zhejiang Province,were identified as Agaricus subrufescens on the basis of sporophore and spore morphology,and ITS sequencing data.Pure cultures of fungal mycelium were obtained from fruit body tissue and confirmed as A.subrufescens on the basis of RAPD data and fruiting tests. |
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| Keywords: | Wild mushroom Agaricus subrufescens isolation of fungal mycelium ITS sequencing |
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