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奶牛体内性控胚胎生产的研究
引用本文:张宝龙,施巧婷,杜坤鹏,王伟伟,闫颖颖,张静芳,王新庄.奶牛体内性控胚胎生产的研究[J].河南农业科学,2009(6).
作者姓名:张宝龙  施巧婷  杜坤鹏  王伟伟  闫颖颖  张静芳  王新庄
作者单位:1. 河南农业大学,牧医工程学院,河南,郑州,450002
2. 河南省农业科学院,畜牧兽医研究所,河南,郑州,450002
3. 西北农林科技大学,动物医学院,陕西,杨凌,712100
基金项目:国家农业科技成果转化资金项目 
摘    要:为探讨奶牛合理的超排方法,采用分离的X冻精对17头超数排卵的荷斯坦奶牛进行人工授精,生产体内性控胚胎。结果表明,处理Ⅰ组在头均获卵数、可用胚及退化胚几个方面都低于处理Ⅱ组,但差异不显著(P>0.05);头均黄体数和未受精数,Ⅰ组高于Ⅱ组(P>0.05);青年牛头均获卵数、可用胚和退化胚方面都高于经产牛组,但差异不显著(P>0.05);青年牛头均未受精卵数显著高于经产牛(P<0.05),头均黄体数低于经产牛,但差异不显著(P>0.05)。

关 键 词:奶牛  超数排卵  X冻精  性控胚胎  可用胚

Study on the Production of Sexing-embryo in vivo for Dairy Cows
ZHANG Bao-long,SHI Qiao-ting,DU Kun-peng,WANG Wei-wei,YAN Ying-ying,ZHANG Jing-fang,WANG Xin-zhuang.Study on the Production of Sexing-embryo in vivo for Dairy Cows[J].Journal of Henan Agricultural Sciences,2009(6).
Authors:ZHANG Bao-long  SHI Qiao-ting  DU Kun-peng  WANG Wei-wei  YAN Ying-ying  ZHANG Jing-fang  WANG Xin-zhuang
Abstract:In order to approach possible superovulation method,17 superovulated holstein cattles were inseminated with X-sorted sperm to product sexing-embryos in vivo.The result showed that the average number of embryos collected per cattle,useful embryos and degraded eggs of treatment group Ⅰ is lower than that of group Ⅱ,but no significant difference was observed(P>0.05).The number of corpus luteum and unfertilizaed eggs of treatment group Ⅰ is higher than that of group Ⅱ(P>0.05).For young cattles,the numbers of embryos,useful embryos and degraded eggs are higher than that of multiparaous cows with no significant difference(P>0.05),while the unfertilizaed eggs of group Ⅰ was significant different from that of group Ⅱ(P<0.05).Additionally,the corpus luteum of young cattles was lower than that of multiparaous cows with no significant difference(P>0.05).
Keywords:Dairy cattle  Superovulation  X-sorted sperm  Sexing-embryos  Useful embryos
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