茄子抗黄萎病遗传的初步研究

选取６份对黄萎病具有抗感差异的茄子材料，利用完全双列杂交设计轮配法配制１５个组合，调查人工灌根后的抗病指数。结果表明：抗性遗传不符合“加性－显性”模型，存在上位性作用，且至少受２对显性基因组控制。

Preliminary Study on the Heredity of the Resistance to Verticillium Wilt in Eggplant

AIM:To establish the cell lines whose hREV3 gene expression was blocked by antisense RNA and observe their characteristis of cell growth rate and N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) sensitivity.METHODS:With modified calcium phosphate-DNA coprecipitation method the eukaryocytic expression plasmid expressing antisense fragment of hREV3, pBK-RSV-hREV3^- and pMAMneo-amp^-hREV3^- were transfected into human embryo kidney cell line of HEK-293. After G418 selection,cell lines of 293-B-hREV3^- and 293-M-hREV3^- were established. By cell counting method, the cell growth rate and MNNG sensitivity of these cell lines were characterized.RESULTS:No change of cell growth rates of these cell lines was observed whether hREV3 gene expression was blocked by either the persistent or induced expression of the antisense hREV3 RNA. While the sensitivity of these cell lines to MNNG was somewhat elevated,as compared with their parent cell line 293 and the cell lines transfected with vector DNAs. CONCLUSION: The gene product of hREV3 was not essential for the cell growth, but it may play a role in the DNA repair functions of the cells after exposure to DNA damaging agents.