Untersuchungen zur Charakterisierung des Ernährungssystems von Karottengewebekulturen: Photosynthese,Kohlenstoff- und Stickstoffernährung,Mineralstoffverbrauch

Characterization of the nutritional system of carrot tissue cultures: Photosynthesis, carbon and nitrogen nutrition, uptake of mineral nutrients During a culture period of 28 days qualitative and quantitative changes in the nutritional system of carrot tissue cultures can be observed. Investigations on sugar uptake, CO₂ fixation, and uptake of amino acids and nitrate indicate a succession of 3 periods, i.e. a heterotrophic period at the beginning of the culture period (up to 10 days), a mixotrophic period (till about the 20th day) followed by an autotrophic period. The transition into the autotrophic period could be only observed in treatments supplemented with kinetin. Uptake of potassium, phosphorus, calcium and magnesium was closely correlated to cell division activity of the explants as induced by m-inositol, IAA and kinetin.