|
|||||
|
|
Analyses of monoclonal antibodies reacting with porcine CD3: results from the Second International Swine CD Workshop |
|
| Authors: | M. D. Pescovitz B. K. Book B. Aasted J. Dominguez A. Ezquerra I. Trebichavsky B. Novikov I. Valpotic J. Nielsen S. Arn D. H. Sachs J. K. Lunney P. C. Boyd J. Walker R. Lee G. Lackovic P. Kirkham R. M. E. Parkhouse A. Saalmü ller |
| Affiliation: | a Indiana University, Department of Surgery and Microbiology/Immunology, Indianapolis, IN 46202, USA b Royal Veterinary and Agricultural University, Frederiksberg C, Denmark c Centro de Investigaciones en Sanidad Animal, INIA, Valdeolmos, Madrid, Spain d Institute Microbiology, Prague, Czech Republic e All-Russian Research Institute of Veterinary Virology and Microbiology, Vlasimizskaya obl., Russia f Department of Biology, Veterinary Faculty, University of Zagreb, Zagreb, Croatia g Danish Veterinary Institute for Virus Research, Lindholm, Kalvehave, Denmark h Transplantation Biology Research Center, Massachusetts General Hospital, Charlestown, MA, USA i Immunology and Disease Resistance Laboratory, LPSI, ARS, USDA, Beltsville, MD, USA j Center for Animal Biotechnology, University of Melbourne, Victoria, Australia k Department of Zoology, Faculty of Sciences, University of Zagreb, Zagreb, Croatia l Institute for Animal Health, Pirbright Laboratory, Surrey, UK m Federal Research Center for Virus Diseases of Animals, Tübingen, Germany |
| Abstract: | Among the 57 monoclonal antibodies (mAb) analyzed within the T-cell group from the Second Swine CD Workshop, six mAb fell within clusters T10 and T11 (No. 088, STH164; No. 148, FY1A3; No. 149, FY2C1; No. 150, FY1H2; No. 151, FY2A11; No. 169, BB23-8E6). The mAb within these two groups gave a similar appearance on flow cytometry and stained all peripheral blood T-cells as defined by CD4 and wCD8 staining. All six mAb precipitated a 24 kDa protein. On the basis of inhibition analyses performed as part of the workshop and from published data, the mAb define at least three epitopes. There is only minimal stimulation of resting peripheral lymphocytes, but four of the mAb produce strong stimulation in the presence of PMA. With the exception of STH164, all have been shown to react with CD3-transfected COS cells. The new mAb, therefore, react with three epitopes on porcine CD3 designated CD3a (BB23-8E6, FY2A11), CD3b (FY1A3, FY2C1), and CD3c (FY1H2). mAb STH164 appears to be reactive with another epitope, however, since its reactivity with CD3 has not been confirmed it is designated as wCD3. |
| Keywords: | Monoclonal antibodies Resting peripheral lymphocytes Epitope Porcine CD3 |
| 本文献已被 ScienceDirect 等数据库收录！ | |