Plasmid transfer between introduced and indigenous bacteria in leaf litter, soil and vermicompost as affected by soil invertebrates |
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Authors: | B A Byzov H Claus E B Tretyakova N F Ryabchenko I N Mozgovaya D G Zvyagintsev Z Filip |
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Institution: | (1) Department of Soil Biology, Faculty of Soil Science, Moscow Lomonosov State University, Vorobjevy Gory, SU-119899 Moscow, Russia, RU;(2) Centre "Bioengineering", RAS, Moscow, Russia, RU;(3) Umweltbundesamt, Institut für Wasser-, Boden- und Lufthygiene, Paul-Ehrlich-Strasse 29, D-63225 Langen, Germany Fax: +49-6103704147, DE |
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Abstract: | Plasmid transfer between introduced strains of Pseudomonas stutzeri JM302 (pLV1013), Pseudomonas putida PAW340 (pLV1017), Pseudomonas aeruginosa PAO5 (RP4), and Enterobacter cloacae MF10 (RP4), all of them harbouring genetically modified or naturally occurring plasmids and bacteria indigenous to oak leaf
litter, soddy-podzolic soil or vermicompost was monitored using non-sterile laboratory microcosms inhabited by the millipede
Pachyiulus flavipes, the woodlouse Armadillidium vulgare, or the earthworms Aporrectodea caliginosa and Eisenia fetida, respectively. Plasmid transfer from genetically modified Streptococcus faecalis JH2-2 (pAMβ1) to Bacillus thuringiensis var. israelensis 1-5 under similar conditions was also followed. A recombinant conjugative plasmid (pLV1017) encoding resistance to antibiotics
and expressing catechol 2,3-dioxygenase was transferred into two facultatively anaerobic gram-negative bacteria, isolated
from the excrement of A. vulgare. Presumed transconjugants of the facultatively anerobic gram-negative bacteria given above were isolated from the leaves
and soil (four strains) and the excrement of the invertebrates (11 strains). They carried the same plasmids and exhibited
the same enzymatic activity as the respective inoculant strains; for most of them, however, DNA fingerprints were slightly
different. Transfer from S. faecalis JH2-2 to B. thuringiensis var. israelensis of a naturally occurring plasmid, pAMb1, encoding resistance to erythromycin was observed in vermicompost.
Received: 18 November 1997 |
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Keywords: | Genetically modified bacteria Soil invertebrates Plasmid transfer Pseudomonas spp Enterobacter cloacae |
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