| 簇毛麦1V染色体SSR标记的筛选 |
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| 引用本文： | 刘守斌,唐朝晖,尤明山,李保云,宋建民,刘广田.簇毛麦1V染色体SSR标记的筛选[J].作物学报,2004,30(2):138-142. |
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| 作者姓名： | 刘守斌 唐朝晖 尤明山 李保云 宋建民 刘广田 |
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| 作者单位： | 中国农业大学作物学院植物遗传育种系，北京 100094 |
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| 基金项目： | 国家自然科学基金，北京市自然科学基金 |
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| 摘 要： | 选用位于普通小麦1A、1B、1D染色体上的32对微卫星引物对普通小麦中国春、簇毛麦、6个中国春-簇毛麦二体附加系和1个普通小麦-簇毛麦二体代换系进行SSR分析，发现引物Xgwm498在簇毛麦中扩增出2条长分别为110 bp和190 bp的片段（即Xgwm498/110和Xgwm498/190）, 这两个片段仅在簇毛麦、中国春-簇毛麦1Ha附加系中出现，其余2Ha、
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| 关 键 词： | 簇毛麦 SSR 染色体特异的SSR标记 |
| 收稿时间： | 2002-09-18 |
| 修稿时间： | 2002年9月18日 |
Characterization of Chromosome 1V Specific SSR Molecular Markers from Haynaldia villosa |
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| LIU Shou-Bin ,TANG Zhao-Hui ,YOU Ming-Shan ,LI Bao-Yun ,SONG Jian-Min ,LIU Guang-Tian.Characterization of Chromosome 1V Specific SSR Molecular Markers from Haynaldia villosa[J].Acta Agronomica Sinica,2004,30(2):138-142. |
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| Authors: | LIU Shou-Bin TANG Zhao-Hui YOU Ming-Shan LI Bao-Yun SONG Jian-Min LIU Guang-Tian |
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| Institution: | College of Crop Science, China Agricultural University, Beijing 100094;2.Xinyang Agricultural College, Xinyang 464000,Henan |
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| Abstract: | Thirty-two SSR primer pairs, distributed in chromosome 1A, 1B and 1D of Triticum aestivum , were investigated on common wheat Chinese Spring, H. villosa, six addition lines of H. villosa chromosome in CS and substitution line 3V of H. villosa chromosome in T. aestivum . Two H. villosa 1V chromosome-specific polymorphic DNA fragment amplified by primer pair Xgwm498, designated Xgwm498/110 and Xgwm498/190 respectively, was obtained from 1V addition line of H. villosa chromosome in CS and H. villosa . There were not Xgwm498/110 and Xgwm498/190 in CS, substitution line 3V and other addition lines of H. villosa chromosome in CS. Therefore, Xgwm498/110 and Xgwm498/190 could be used as two molecular markers for detection of chromosome 1V of H. villosa in common wheat. |
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| Keywords: | SSR |
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