Changes of MARCKS mRNA expression in rat hippocampus with acute multi-cerebral infarction

AIM: To observe the dynamic alteration of myristoylated alanine-rich C kinase substrate （MARCKS） mRNA expression in rat hippocampus with acute multi-cerebral infarction, and discuss the relationship between the alteration of hippocampus MARCKS gene and ischemia damage. METHODS: The acute multi-cerebral infarction model was established by method of Kaneko. Neurological function deficits were evaluated in the behavior test. The consequences of cerebral ischemic damage were examined by histopathological analyses. The MARCKS mRNA expression was measured by semi-quantitative PCR. RESULTS: The rats in acute multi-cerebral infarction group showed different level changes of neurological function deficits. The hippocampus damage of histopathology became significant 24h after ischemia. At the same time, the MARCKS mRNA expression was upregulated at the area of rats hippocampus during ischemia, and its overexpression started 1h after ischemia, and reached maximum7d after ischemia. CONCLUSION: MARCKS mRNA of rat hippocampus overexpresses during acute cerebral ischemia. This MARCKS mRNA overexpression is related with hippocampus ischemia damage.