Inhibition of proliferation in B lymphoma cell line Raji transfected with exogenous HCAP1 gene |
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Authors: | GUO Lie-ping WANG Rong WU Xiang-hua DU Jun-xiang MU Qi-tian SUN Fen-yong WAN Da-fang GU Jian-ren |
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Affiliation: | 1.Institute of Hematology, Medical College,2Institute of Biological Engineering, Jinan University, Guangzhou 510632, China;3National Laboratory for Oncogene and Related Gene, Shanghai Cancer Institute, Shanghai 200032, China |
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Abstract: | AIM: HCAP1 gene has been mapped at human chromosome band 17p13.3, a region with high frequency of loss of heterzygosity in various types of tumor. The aim of this study was to investigate the effects of exogenous HCAP1 gene products on proliferation of B lymphoma cell line Raji. METHODS: HCAP1 gene was transfected into Raji cells. The cells stably expressed exogenous HCAP1 gene were screened with G418. The effects of HCAP1 protein overexpression on proliferation in Raji cells were assessed by viable cell count, cell growth curve, colony formation assay in soft agar, and 5-bromo-2'-deoxy-uridine (BrdU) labeling and detection assay. RESULTS: The Raji cells overexpressed HCAP1, displayed significantly slow growth rate, extended cell doubling time, decreased capacity of colony formation and reduced percentage of BrdU incorporation in the cells (P<0.05), as compared with control Raji cells which transfected with empty vector, or compared with parent Raji cells. CONCLUSION: Overexpression of exogenous HCAP1 in Raji cells inhibits the cell proliferation. |
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