Induction of stem cells from adult Beagle canine bone marrow into chondrocytes in vitro

AIM：To explore the methods of the differentiation from adult Beagle canine bone marrow stem cells (BMSCs) into chondrocytes in vitro and determine the factors involved in the differentiation process.METHODS：About 10 mL BMSCs were aspirated from canine femoral bone,primarily cultured and subcultured in vitro.TGF-β1 was added into the culture medium.BMSCs were cultured and expanded in the medium until they reached the required quantity.BMSCs were induced to differentiate into chondrocytes at high cell density.Matrix of cartilage cells was detected by toludine blue stain,and cartilage specific collagen Ⅱ was detected by immunohistochemistry.RESULTS：The structure of cellular cartilage form BMSCs was uniformly positive of toludine blue staining.Immunohistochemical staining was positive for the collagenⅡ.CONCLUSION：Application of TGF-β1 may induce canine bone marrow stem cells into chondrocytes in vitro,which can be used as seeding cells in cartilage tissue engineering.