Antitumor effects of HSV-tk and cytosine deaminase double suicide gene mediated by adenovirus on cholangiocarcinoma cells in vitro |
| |
| Authors: | LI Mei-sheng LIANG Li-jian CHU Zhong-hua HUANG Jie-fu ZHEN Zuo-jun |
| |
| Affiliation: | 1.Department of Hepatobiliary Surgery, The First People's Hospital of Foshan, Foshan 528000, China;2.Department of Hepatobiliary Surgery, The First Affiliated Hospital of Sun Yet-sen University, Guangzhou 510080, China;3.Department of Surgery, The Second Affiliated Hospital of Sun Yet-sen University, Guangzhou 510120, China；4Ministry of Public Health of China, Beijing 100004, China |
| |
| Abstract: | AIM: To study the antitumor effects of HSV-tk and cytosine deaminase double suicide gene system on cholangiocarcinoma cells in vitro. METHODS: Recombinant adenovirus vector carring HSV-tk and cytosine deaminase double suicide gene was constructed and was transfected into QBC939 huamn cholangiocarcinoma cells. The antitumor effects were observed in vitro in comparison with a single suicide gene system. RESULTS: By transfection, HSV-tk and (or) cytosine deaminase double suicide gene gained high expression in tumor cells. Compared with single suicide gene system, this double gene system presented higher sensitivity, stronger antitumor effect and bystander effect. CONCLUSION: HSV-tk and cytosine deaminase double suicide gene system has a powerful antitumor effect on cholangiocarcinoma cells. |
| |
| Keywords: | |
|
| 点击此处可从《园艺学报》浏览原始摘要信息 |
|
点击此处可从《园艺学报》下载全文 |
|
