The immunity effect of B7-H1 blockade on immature dendritic cells |
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Authors: | LI Dong-yin c CHU Zhong-hua WAN Yun-le SHAO Jing WEI Jing LIU Shan-ying ZHENG Li-min OU Qing-jia |
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Institution: | 1.Department of Hepatic-Biliary Surgery,2Research Center of Medicine, The Second Affiliated Hospital of Sun Yat-sen University, Guangzhou 510120, Chian;3Life Science College, Sun Yat-sen University, Guangzhou 510275, Chain |
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Abstract: | AIM: To investigate the immune stimulation capacity of B7-H1 blockade on immature dendritic cells (DCs) in vitro. METHODS: The human monocyte-derived dendritic cells were induced in the presence of cytokine GM-CSF and IL-4. The expression of B7-H1 was detected by FCM. On blockade of B7-H1, the maturation and endocytic activity, T cells stimulatory proliferation capacity, IL-12 production, T cell differentiation effect of DCs were detected by FCM, MTT assay, ELISA and ELISPOT, respectively. RESULTS: The expression of B7-H1 was increased with the induction of DCs. On day 7, the positive expression was 54.12%, and the TNF-α induced mature DCs had the positive expression rate of 83.64%. The blockade of B7-H1 on immature DCs had sharply increased their T cells stimulatory proliferation capacity and IL-12 production, and efficiently induced the development of Th1/Tc1 cells, but had no effect on their maturation and endocytic activity. CONCLUSION: The blockade of B7-H1 on immature DCs increases its immune stimulation activity. It is valuable to investigate the antitumor immune responses of DCs vaccine with B7-H1 blockade. |
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