Effects of chronic hypoxia on intracellular calcium concentration in pulmonary arterial smooth muscle cells |
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Authors: | WU Hui-min XU Yong-jian ZHANG Zhen-xiang XU Shu-yun |
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Institution: | Department of Respiratory Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China |
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Abstract: | AIM:To study the effect of chronic hypoxia (CH) on the intracellular calcium ([2+]i) in pulmonary artery smooth muscle cells (PASMCs) and the role of L-type calcium channel and calcium store. METHODS:The rat chronic hypoxia model was set up and intervene the PASMCs with normal PSS, calcium-free PSS, nifedipine, and heparine respectively. The resting [Ca2+]i was determined with the Fura-2/AM calcium imaging technique. RESULTS:(1) The [Ca2+]i in CH group in normal PSS was higher than that in control group in normal PSS (P<0.05). The [Ca2+]i in CH group in normal PSS was higher than that in calcium-free PSS (P<0.05). (2) No obvious change of [Ca2+]i before and after application of nifedipine in PASMCs of CH groups was observed. (3) No difference of [Ca2+]i before and after application of heparine in PASMCs of CH groups was detected. CONCLUSION:Chronic hypoxia increased the [Ca2+]i in PASMCs. Chronic hypoxia induced increase in [Ca2+]i may relate to the influx of extracellular calcium, but not due to the L-type calcium channel or the IP3R modulation only. |
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