Influence of Weimaining on the cell cycle of murine Lewis lung carcinoma

AIM:To investigate the anti-tumor effect of Weimaining (WMN) on a murine Lewis lung carcinoma (3LL) and the influence on the cell cycle. METHODS:The inhibitory rate of WMN in 3LL growth was detected by replicating the model of 3LL. The effect of the drug on 3LL cell cycle and the influence of the drug on the expression of cyclin D1 protein were investigated by flow cytometry and immunohistochemical staining. RESULTS:The results showed that the inhibitory rate of drug in 3LL is 19.14%, 33.59%, 40.63% and 51.56% respectively at dosage ranging from 100,150, 200 and 250 mg·kg^-1·d^-1. The drug inhibits tumor growth in a dose-dependent manner. The drug arrests 3LL cells in G₀-G₁ phase and decreases the expression of cyclin D1 protein. CONCLUSION:WMN inhibits the growth of 3LL cells in vivo by decreasing the expression of cyclin D1, blocking the cells in G₀-G₁ phase and preventing the cells transition from G₁ to S phase while DNA is replicated.