Effects of human recombinant acidic fibroblast growth factor on cell proliferation and apoptosis in NIH3T3 cells

AIM:Through studying the differences betwe en wild-type acidic fibroblast growth factor (waFGF) with recombinant aFGF (raFG F ),the biological effect of raFGF concerned with mitogenic activity was evaluate d.METHODS:NIH3T3 cell line was used.Cell proliferation with MTT method was used to study the mitogenic activity.Flow cytometry was also used fo r detection of apoptosis,cell membrane permeability and mitochondria potential.The role of heparin sulfate (HS) on aFGF biological effect was studied at the s ame time in this research.RESULTS:The enhancement of raFGF on cell proliferation was sign ificantly lower than that of waFGF.The restriction of raFGF on apoptosis and th e enhancement of it on cell membrane permeability were all lower than those of w aFGF significantly.The enhancement of raFGF on mitochondria potential was lower than that of waFGF significantly.The HS improved the biological effect of aFGF .CONCLUSION:The mitogenic activity of raFGF is lower than that of waFGF and raFGF has little effect on apoptosis.