Expression of tissue factor in cerebral microvascular thrombosis in rats

AIM: To study the expression of tissue factor (TF) in cerebral microvascular thrombosis and its dynamic changes in rats. METHODS: 50 female SD rats were randomized to control group, 2, 4, 6, and 24 hours after thrombosis groups, 10 rats in each group. The model of cerebral microvascular thrombosis was induced by photo-chemical method. ELISA and immunohistochemistry methods were used to observe the changes of TF contents in blood plasma and the expression of TF in cerebral microvascular in each group. RESULTS: Cerebral thrombosis was induced by photo-chemical method successfully. The TF content in plasma was obviously higher in 4 h and 6 h groups than that in control group (P＜0.01). TF intensely expressed on vascular endothelial cells in thrombosis groups and negatively expressed in control group. The average A value of TF in 6 h group and 24 h group was significantly higher than that in 2 h and 4 h groups (P＜0.01). It was also found that vWF content in plasma was obviously higher in 2 h group (P＜0.01) and 4 h group (P＜0.05) group than that in control group. AT activity in plasma showed obviously increased in 24 h group, compared with that in the others (P＜0.05). CONCLUSION: These findings suggest the photochemical infarct model is convenient to investigate cerebral thrombosis. TF plays an important role in thrombosis in this model.