NT-3 genetically modified Schwann cells promote neural stem cells to differentiate into neuron-like cells |
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Authors: | GUO Jia-song ZENG Yuan-shan LI Hai-biao HUANG Wen-lin LIU Ran-yi |
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Affiliation: | 1.Division of Neuroscience, Department of Histology and Embryology, Zhongshan Medical College,2Cancer Center, Sun Yat-sen University, Guangzhou 510080, China;3.Branch Campus of The First Military Medical University,Guangzhou 510315, China |
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Abstract: | AIM: To explore the effects of neurotrophin-3 (NT-3)-genetically modified Schwann cells (NT-3-SCs) on differentiation of neural stem cells (NSCs) into the neuron-like cells. METHODS: The NSCs were co-cultured with NT-3-SCs. Report gene LacZ genetically modified Schwann cells (LacZ-SCs) and normal SCs respectively in vitro. 7 d later, the differentiation of NSCs was studied by immunohistochemistry, and the percentage of neuron-like cells was calculated. RESULTS: NSCs differentiated to the GFAP-positive cells (glial-like cells) and NF-positive cells (neuron-like cells) in vitro. Compared to the normal SCs, NT-3-SCs more efficiently promoted NSCs to differentiate into the neuron-like cells. The effect of LacZ-SCs was as the same to the normal SCs. CONCLUSION: NT-3-SCs promote NSCs to differentiate into the neuron-like cells. |
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