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Norcantharidin induces HeLa cells apoptosis through caspases pathway
Authors:AN Wei-wei  WANG Min-wei  GONG Xian-feng  TASHIRO Shin-ichi  ONODERA Satoshi  IKEJIMA Takashi
Institution:1.China-Japan Research Institute of Medical and Pharmaceutical Sciences, 2Department of Pharmacology, Shenyang Pharmaceutical University, Shenyang 110016, China;3.Department of Clinical and Biomedical Sciences, Showa Pharmaceutical University, Tokyo 19-8543, Japan
Abstract:AIM: To examine the apoptotic pathway of norcantharidin (NCTD)-induced HeLa cells death. METHODS: MTT, photomicroscopical observation, DNA agarose gel electrophoresis, LDH release and Western blot analysis were used. RESULTS: NCTD induced HeLa cells apoptosis and the apoptosis was partially reversed by the inhibitors of caspase-family (-3, -8, -10). The activities of caspase-3, -8 and -9 were significantly increased after treated with NCTD. The expression of the inhibitor of caspase-3 activated DNase (ICAD) was decreased in a time dependent manner. CONCLUSION: NCTD induces HeLa cells apoptosis through activating caspase pathways.
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