Effect of pregnancy uterine microenvironment on the expression of NKG2A,NKG2D and their ligands in decidual NK cells |
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Authors: | ZHAO Li-li QU Xun LIANG Lu YANG Mei-xiang KONG Bei-hua DONG Bai-hua LV Xiao-mei ZHANG You-zhong BAI Xiao-xia |
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Institution: | 1.Basic Medical Science Institute,2 Department of Obstetrics and Gynecology,Qilu Hospital,Shandong University,3 Shandong Academy of Medical Sciences,Jinan 250012,China |
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Abstract: | AIM:To investigate the expression of NKG2A,NKG2D and their ligands in pregnancy uterine micro-environment and to probe the function of NKG2A and NKG2D imbalance expression during the immunotolerance at the fetal-maternal boundary.METHODS:Decidual lymphocytes and peripheral lymphocytes were obtained from 30 women during 6-9 weeks of pregnancy who were undergoing selective termination.FACS technology was used to detect NK cells number and NKG2A,NKG2D expression.RT-PCR was used to investigate HLA-E and MICA mRNA in trophoblast tissue.RESULTS:Natural killer cells predominate,accounting for 70% of pregnancy endometrial lymphocytes.FACS results indicated that NKG2A was significantly increased in decidual NK cells as compared with that in peripheral NK cells,accounting for 97.86%±1.75% and 33.35%±10.92%.The difference between them in NKG2A expression was significant (P<0.05).Although the levels of NKG2D in decidual NK cells were similar to that in peripheral NK cells,accounting for 93.21%±4.52% and 97.80%±1.72%,respectively,the difference between them in NKG2A expression was also significant (P<0.05).At the same time,we found HLA-E mRNA in the trophoblast tissue.There was no proof yet on the expression of NKG2D ligand-MICA on trophoblast.CONCLUSIONS:Natural killer cells are the predominant lymphocytes during the first trimester of decidual tissue.These cells have a great different antigenic phenotype with peripheral natural killer cells.Decidual natural killer cells have high expression of NKG2A and trophoblast tissue expressed HLA-E.This may be an important factor contributing to the immunotolerance at the fetal - maternal boundary during pregnancy. |
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Keywords: | Decidua Killer cells natural Pregnancy |
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