| Abstract: | AIM:To investigate the role of D1 and D3 d opamine receptor on MAPK signal transduction and c-fos gene expression after acute cocaine treatment.METHODS:Activations of extracellular signal-regulated kinase (E RK),the c-Jun N-terminal kinase (JNK),p38 activation and expression of c-fos in wild type and D1 and D3 receptor mutant mice after acute cocaine treatment were checked by Western blotting.RESULTS:ERK activation and c-fos induction was enhanced in D3 mutant mice and abolished in D1 mutant mice by acute cocaine treatment,while p38 and JNK activation was not obviously modulated by the D1 and D3 receptors b y acute cocaine treatment.Meanwhile,c-fos induction was inhibited when SL3 27,a specific MEK inhibitor,was injected before cocaine treatment.CONCLUSION:D1 and D3 receptors play opposite roles in the regul ation of ERK activation and c-fos gene expression after acute cocaine treatm ent.The expression of c-fos gene depends on ERK signal pathway after acute cocaine treatment. |
