Peroxisome proliferator-activated receptor γ agonist attenuates ICAM-1 and CINC-1 expression in lungs of rats with acute lung injury |
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Authors: | LIU Dong ZENG Bang-xiong ZHANG Shi-hai GENG Zhi-long ZHANG Shi-fan |
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Institution: | 1.Department of Anesthesiology,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China;2.Department of Anesthesiology,Lanzhou General Hospital of PLA,Lanzhou 730050,China |
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Abstract: | AIM:To investigate the effects of rosiglitazone (ROSI),an agonist of peroxisome proliferator-activated receptor γ (PPARγ),on the lung expression of intercellular adhesion molecule-1 (ICAM-1) and cytokine-induced neutrophil chemoattractant (CINC) in rats with acute lung injury.METHODS:Thirty-six male Wistar rats were randomly divided into six groups:control group,ROSI group,GW9662 (a PPARγ antagonist) group,lipopolysaccharide (LPS,6 mg/kg,iv) group,ROSI-LPS group (0.3 mg/kg ROSI iv 30 min prior to LPS) and GW9662-ROSI-LPS group (0.3 mg/kg GW9662,iv,20 min before ROSI).Four hours after LPS injection,wet/dry weight (W/D) ratio,myeloperoxidase (MPO) activity,malondialdehyde (MDA) and CINC-1 concentrations were assayed in the lung tissues.Immunohistochemical analysis of ICAM-1 expression was also studied.RESULTS:Pretreatment with ROSI significantly attenuated LPS-induced increases in W/D ratio,MPO activity,MDA and CINC-1 concentrations as well as ICAM-1 expression in the lung tissues.The specific PPARγ antagonist GW9662 antagonized the effects of ROSI.CONCLUSION:Pretreatment with ROSI reduces LPS-induced lung injury in rats.The mechanism involves inhibition of the lung expression of ICAM-1 and CINC-1 by the activation of PPARγ. |
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