Construction of recombinant adenoviruses expressing the helicobacter pylori CagA antigen |
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Authors: | WANG Xuan LIU Jin-bao ZHAO Xiao-dong WU Shu-hua DONG Wei-hua LU Li |
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Institution: | 1.Department of Pathophysiology, Guangzhou Medical College, Guangzhou 510182, China;2.State Key Laboratory of Molecular Virology and Genetic Engineering,Institute of Virology, Chinese Center of Disease Control and Prevention, Beijing 100052, China |
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Abstract: | AIM: To construct a replication-defective recombinant adenovirus, which expresses the CagA gene. METHODS AND RESULTS: The CagA gene was amplified by PCR. This heterogeneous gene was cloned into shuttle vector pAdTrack-CMV. The recombinant adenovirus DNA was obtained by the homologous recombination between the shuttle vector and adenovirus DNA in E.coli 5183. After linearization, the recombinant adenovirus DNA was transfected into 293 cells and the recombinant adenovirus was obtained. Through this technique, the replication- defective recombinant adenovirus AdEasyCagA was constructed. CONCLUSIONS: The replication-defective recombinant adenoviruses AdEasyCagA was constructed successfully. The work will make a good foundation for studying the effects of the replication-defective recombinant adenovirus on Th1/Th2 balance in asthma and be useful for finding a new pathway to prevent and cure asthma. |
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