The protection of composite salviae dropping pills against HUVECs injure induced by H2O2

AIM: To observe the protective effect of composite salviae dropping pills against human umbilical vein endothelial cells (HUVECs) injure induced by hydrogen dioxide (H2O2) and discuss the mechanism of its therapeutic action on cardiac and encephalic diseases. METHODS: HUVECs were injured by 0.1 mmol/L H2O2 and then different final concentrations of composite salviae dropping pills (0.5 g/L, 0.25 g/L, 0.1 g/L) were added before and after the injury. Cell viability was measured by MTT assay. TBA method was used to detect the intracellular malonaldehyde. Nitric oxide (NO) in the culture medium was detected by using nitrate reductase assay, and immunocytochemisty was used to observe the expression of NOS2, NOS3 and NF-κB. Morphologic observation was also performed. RESULTS: HUVECs were injured by 0.1 mmol/L H2O2. Composite salviae dropping pills increased the cell viability apparently, inhibited the production of MDA induced by H2O2, regulated the generation of NO bilaterally and influenced the expression of NOS3 and NF-κB. CONCLUSIONS: Composite salviae dropping pills at concentrations of 0.5 g/L, 0.25 g/L and 0.1 g/L protects HUVECs from injury by H2O2, no matter it is be added before or affter the injury. The possible mechanism is associated with its regulating the expression of NOS2, NOS3 and NF-κB.