| CTAB-silica Method for DNA Extraction and Purification from Castanea mollissima and Ginkgo biloba |
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| 引用本文： | ShenYongbao ShiJisen. CTAB-silica Method for DNA Extraction and Purification from Castanea mollissima and Ginkgo biloba[J]. 中国林学(英文版), 2003, 5(3): 10-12 |
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| 作者姓名： | ShenYongbao ShiJisen |
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| 作者单位： | NationalSouthernTreeSeedInspectionCenter,NanjingForestryUniversity,Nanjing210037,P.R.China |
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| 摘 要： | A new method CTAB-silica for DNA extraction and purification from the leaves and buds of Castanea mollissima and Ginkgo biloba was tested. The method is based on the silica-based purification protocol developed by Boom et al. (1990). By modifying the protocol, plant genome DNA could be extracted easily from dormant buds, mature leaves, and other parts of plant. Our results showed that the purified DNA was of high purity and could be analyzed by PCR. Furthermore, this CTAB-silica method took much less time for a successful DNA purification process compared to the traditional methods (CTAB and SDS). By our method,the suitable DNA can be extracted and purified from over 10 plant samples by one person in an hour.
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| 关 键 词： | 银杏 栗 DNA 提取 纯化 溴化十六烷三甲基铵-硅酸法 |
CTAB-silica Method for DNA Extraction and Purification from Castanea mollissima and Ginkgo biloba |
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| Shen Yongbao,Shi Jisen. CTAB-silica Method for DNA Extraction and Purification from Castanea mollissima and Ginkgo biloba[J]. Forestry Studies in China, 2003, 5(3): 10-12 |
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| Authors: | Shen Yongbao Shi Jisen |
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| Abstract: | A new method CTAB-silica for DNA extraction and purification from the leaves and buds of Castanea mollissima and Ginkgo biloba was tested. The method is based on the silica-based purification protocol developed by Boom et al. (1990). By modifying the protocol, plant genome DNA could be extracted easily from dormant buds, mature leaves, and other parts of plant. Our results showed that the purified DNA was of high purity and could be analyzed by PCR. Furthermore, this CTAB-silica method took much less time for a successful DNA purification process compared to the traditional methods (CTAB and SDS). By our method, the suitable DNA can be extracted and purified from over 10 plant samples by one person in an hour. |
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| Keywords: | DNA extraction and purification CTAB-silica method Castanea mollissima Ginkgo biloba |
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