The preliminary study on the effects of growth hormone and insulin‐like growth factor‐I on κ‐casein synthesis in bovine mammary epithelial cells in vitro |
| |
| Authors: | M Z Wang Y Ji C Wang L M Chen H R Wang J J Loor |
| |
| Institution: | 1. College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu, China;2. School of Clinical Medicine, Jiangsu University, Zhenjiang, Jiangsu, China;3. Department of Animal Sciences and Division of Nutritional Science, University of Illinois, Urbana, IL, USA |
| |
| Abstract: | The effects of growth hormone (GH) and insulin‐like growth factor‐I (IGF‐I) on protein synthesis and gene expression of κ‐casein in bovine mammary epithelial cell in vitro were studied. The treatments were designed as follows: the growth medium without serum was set as the control group, while the treatments were medium supplemented with GH (100 ng/ml), IGF‐I (100 ng/ml), and GH (100 ng/ml) + IGF‐I (100 ng/ml). The quantity of κ‐casein protein was measured by ELISA, and the κ‐casein gene (CSN3) expression was examined by real‐time quantitative PCR (RT‐qPCR). Compared with the control group, all the experimental groups had greater (p < 0.05) expression of CSN3. The concentration of κ‐casein followed a similar response as CSN3, but the difference between the treatments and the control was not statistically significant (p > 0.05). Furthermore, no synergistic effect of GH and IGF‐I was observed for both the κ‐casein concentration and CSN3 expression. It is therefore concluded that GH or IGF‐I can independently promote the expression of CSN3 in bovine mammary epithelial cells in vitro. |
| |
| Keywords: | growth hormone insulin‐like growth factor‐I κ ‐casein protein CSN3 gene expression |
|
|
