|
|||||
|
|
| 高效液相色谱测定复方聚维碘凝胶中氢化可的松的含量 | |
| 引用本文： | 江喜春.高效液相色谱测定复方聚维碘凝胶中氢化可的松的含量[J].中国兽药杂志,2010,44(3):1-4. |
| 作者姓名： | 江喜春 |
| 作者单位： | 1. 华中农业大学动物科技学院,武汉430070;安徽省农业科学院畜牧兽医研究所,合肥230030 2. 安徽省农业科学院畜牧兽医研究所,合肥,230030 3. 华中农业大学动物科技学院,武汉,430070 |
| 基金项目： | 国家科技支撑计划(奶业专项)子项目，农业部公益性行业(农业)科研专项子项目 |
| 摘 要： | 采用高效液相色谱法（HPLC）测定复方聚维酮碘凝胶中氢化可的松的含量。以C18（4.6mm×250mm，5μm）为色谱柱，乙腈-甲醇-醋酸铵（20：45：35）为流动相，流速1mL／min，检测波长240nm，柱温37℃。结果显示：氢化可的松在0．025～0．122mg／mL范围内呈良好的线性关系（r=0．9993），回收率为96．06％，相对标准偏差（RSD）为0．45％。试验表明，采用高效液相色谱法测定复方聚维酮碘凝胶中氢化可的松的含量灵敏、快速、准确，可用于该复方制剂的质量控制或检验分析。 |
| 关 键 词： | 高效液相色谱 氢化可的松 相对标准偏差 测定 |
| 收稿时间： | 2009/6/26 0:00:00 |
| 修稿时间： | 2009/7/31 0:00:00 |
Determination of Hydrocortisone in Compound Povidone Iodine Gel by HPLC |
|
| Jiang Xi-chun and.Determination of Hydrocortisone in Compound Povidone Iodine Gel by HPLC[J].Chinese Journal of Veterinary Drug,2010,44(3):1-4. | |
| Authors: | Jiang Xi-chun and |
| Institution: | Institute of Animal Science and Veterinary Medicine, Anhui Academy of Agricultural Science, |
| Abstract: | Hydrocortisone in compound povidone iodine gel was determined by HPLC with C18 （4.6 mm × 250 mm, 5 μm ） as chromatography column, mobile phase consisting of aeetonitrile ： methanol ： ammonium acetate （20： 45： 35）. The detection wavelength was 240 nm and the flow rate was 1 mL · min^-1 at 37℃ of the column temperature. The results showed that there was a good linear relationship within the hydrocortisone concentration range of 0. 025 - 0. 122 mg · mL^-1（r = 0. 999 3）, and the recovery rate was 96.06% with relative standard deviation （RSD） of 0.45%. The test indicated that the method of HPLC was sensitive, rapid and accurate for detecting the hydrocortisone in compound povidone iodine gel, and it can be used for quality control or check up analysis of this compound preparation. |
| Keywords: | HPLC Hydrocortisone Relative standard deviation (RSD) Determination |
| 本文献已被 维普 万方数据 等数据库收录！ | |
| 点击此处可从《中国兽药杂志》浏览原始摘要信息 | |
| 点击此处可从《中国兽药杂志》下载免费的PDF全文 | |