Development and evaluation of a rapid immunomagnetic bead assay for the detection of classical swine fever virus antigen |
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Authors: | James V Conlan Syseng Khounsy Stuart D Blacksell Christopher J Morrissy Colin R Wilks Laurence J Gleeson |
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Institution: | (1) CSIRO Australian Animal Health Laboratory, Geelong, Victoria, Australia;(2) School of Veterinary Science, The University of Melbourne, Parkville, Victoria, Australia;(3) National Animal Health Centre, Department of Livestock and Fisheries, Ministry of Agriculture and Forestry, Vientiane, Lao People’s Democratic Republic;(4) Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand;(5) Centre for Tropical Medicine, Nuffield Department of Clinical Medicine, Churchill Hospital, University of Oxford, Oxford, UK;(6) ACIAR Project AH/2003/001, P.O. Box 7042, Vientiane, Lao PDR |
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Abstract: | Classical swine fever (CSF) is a highly contagious and severe viral disease of swine resulting in substantial production losses
in different farming systems in many regions of the world. The accurate and rapid detection of CSF outbreaks is reliant on
sensitive and specific laboratory testing and is a key component of disease control. Specific detection of CSF virus can be
achieved by virus isolation in tissue culture, antigen capture or the detection of viral RNA using molecular techniques. In
order to reduce the time taken to achieve a diagnostic result and simplify testing methods, an antigen capture ELISA using
immunomagnetic beads (IMB) as the solid phase was developed and compared to a microplate-based antigen capture (AC)-ELISA.
The IMB-ELISA has up to 64-fold greater analytical sensitivity than the AC-ELISA and initial estimates of diagnostic sensitivity
and specificity are 100%. The IMB-ELISA has a highly robust, rapid and stable test format and is simpler to perform than the
AC-ELISA. The IMB-ELISA has the added advantage that a result can be sensitively and specifically determined by eye, lending
it to the possibility of adaptation to a near-to-field test with minimal equipment or expertise needed. |
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Keywords: | Classical swine fever virus Diagnosis Rapid test Immunomagnetic Bead Microparticle ELISA |
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