| 噬菌体裂解酶Cp51在重组乳酸菌中的表达及对A型产气荚膜梭菌的抗菌活性 |
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| 引用本文： | 龙航宇,陶佳梦,姜亚杰,李晓斌,邹佳汝,翁亚彪,林瑞庆. 噬菌体裂解酶Cp51在重组乳酸菌中的表达及对A型产气荚膜梭菌的抗菌活性[J]. 华南农业大学学报, 2020, 41(1): 42-47. DOI: 10.7671/j.issn.1001-411X.201903003 |
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| 作者姓名： | 龙航宇 陶佳梦 姜亚杰 李晓斌 邹佳汝 翁亚彪 林瑞庆 |
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| 作者单位： | 华南农业大学兽医学院/广东省动物源性人兽共患病预防与控制重点实验室,广东广州510642;华南农业大学兽医学院/广东省动物源性人兽共患病预防与控制重点实验室,广东广州510642;华南农业大学兽医学院/广东省动物源性人兽共患病预防与控制重点实验室,广东广州510642;华南农业大学兽医学院/广东省动物源性人兽共患病预防与控制重点实验室,广东广州510642;华南农业大学兽医学院/广东省动物源性人兽共患病预防与控制重点实验室,广东广州510642;华南农业大学兽医学院/广东省动物源性人兽共患病预防与控制重点实验室,广东广州510642;华南农业大学兽医学院/广东省动物源性人兽共患病预防与控制重点实验室,广东广州510642 |
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| 基金项目： | 广东省重点领域研发计划（2019B020218004）；广东省科技计划（2014A020208099） |
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| 摘 要： | 【目的】构建A型产气荚膜梭菌Clostridium perfringens裂解酶重组表达乳酸菌Lactococcus lactis,并检测重组菌及其表达产物的抗菌效果。【方法】全基因合成噬菌体裂解酶Cp51基因,构建PNZ8148-Cp51原核表达重组载体,电转化至乳酸菌NZ9000,经乳链菌肽诱导表达可溶性Cp51蛋白;用比浊法检测表达蛋白对A型产气荚膜梭菌的抗菌活性;利用细菌液混合培养检测重组菌对A型产气荚膜梭菌增殖的抑制作用。【结果】噬菌体裂解酶Cp51在乳酸菌中成功表达,为1 268 bp;质量浓度1μg·mL-1以上的重组蛋白对A型产气荚膜梭菌具有高效抗菌活性;重组乳酸菌对A型产气荚膜梭菌增殖有一定的抑制效果,混合培养后使产气荚膜梭菌活菌数从9×10~9 CFU·mL~(-1)下降到约9×10~8 CFU·mL~(-1)。【结论】A型产气荚膜梭菌噬菌体裂解酶重组乳酸菌构建成功,为后续优化表达和临床应用研究奠定了基础。
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| 关 键 词： | 产气荚膜梭菌 乳酸菌 裂解酶 重组蛋白 抗菌活性 |
| 收稿时间： | 2019-03-01 |
Expression of phage lysin Cp51 in recombinant Lactococcus lactis and its antibacterial activity against Clostridium perfringens type A |
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| LONG Hangyu,TAO Jiameng,JIANG Yajie,LI Xiaobin,ZOU Jiaru,WENG Yabiao and LIN Ruiqing. Expression of phage lysin Cp51 in recombinant Lactococcus lactis and its antibacterial activity against Clostridium perfringens type A[J]. JOURNAL OF SOUTH CHINA AGRICULTURAL UNIVERSITY, 2020, 41(1): 42-47. DOI: 10.7671/j.issn.1001-411X.201903003 |
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| Authors: | LONG Hangyu TAO Jiameng JIANG Yajie LI Xiaobin ZOU Jiaru WENG Yabiao LIN Ruiqing |
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| Affiliation: | College of Veterinary Medicine, South China Agricultural University/Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou 510642, China,College of Veterinary Medicine, South China Agricultural University/Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou 510642, China,College of Veterinary Medicine, South China Agricultural University/Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou 510642, China,College of Veterinary Medicine, South China Agricultural University/Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou 510642, China,College of Veterinary Medicine, South China Agricultural University/Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou 510642, China,College of Veterinary Medicine, South China Agricultural University/Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou 510642, China and College of Veterinary Medicine, South China Agricultural University/Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou 510642, China |
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| Abstract: | |
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| Keywords: | Clostridium perfringens Lactococcus lactis lysin recombinant protein antibacterial activity |
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