| Abstract: | Upon infection byCladosporium fulvum, tomato plants start to produce pathogenesis-related (PR) proteins. The PR proteins 1,3-β-glucanase, chitinase, and PR-1b
accumulated near the stomata in the lower epidermis ofC. fulvum-inoculated tomato leaves as could be determined by immunolocalization with polyclonal antibodies. However, no differences
in accumulation of PR proteins between a compatible and an incompatible interaction were found. Results obtained from enzyme
activity measurements of 1,3-β-glucanase and chitinase on similar leaf material as used for the immunolocalization did not
fully reflect the immunolocalization data. The antibodies possibly detect only the extracellular but not the intracellular
enzymes. The accumulation of PR proteins near the stomata might be part of a general defence response of plants against pathogens
and potential pathogens. |
