| 桃组织培养和遗传转化研究现状及展望 |
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| 引用本文： | 吴延军,徐昌杰,张上隆.桃组织培养和遗传转化研究现状及展望[J].果树学报,2002,19(2):123-127. |
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| 作者姓名： | 吴延军 徐昌杰 张上隆 |
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| 作者单位： | 1. 浙江大学华家池校区园艺系,浙江杭州,310029;安徽农业大学园艺系,安徽合肥,230036
2. 浙江大学华家池校区园艺系,浙江杭州,310029 |
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| 摘 要： | 现代生物技术如组织培养和遗传转化在植物育种方面有重要作用。综述了桃树的组织培养技术和桃遗传转化研究取得的成绩。文中侧重介绍了广泛应用于桃树研究的茎尖培养法、胚培养法两种主要的组织培养技术和农杆菌介导法、基因枪轰击法两种主要的遗传转化方法;详细说明了品种、外植体类型及其发育阶段、培养基类型、生长调节因子和培养环境等对桃组织培养的影响;初步分析了影响桃树遗传转化的关键问题,并对进一步的工作重点提出了建议。
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| 关 键 词： | 桃 组织培养 遗传转化 |
| 文章编号： | 1009-9980-(2002)-02-123-05 |
| 修稿时间： | 2001年5月20日 |
Status and Prospect of Research in Peach Tissue Culture and Genetic Transformation |
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| Wu Yanjun ,Xu Changjie ,and Zhang Shanglong.Status and Prospect of Research in Peach Tissue Culture and Genetic Transformation[J].Journal of Fruit Science,2002,19(2):123-127. |
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| Authors: | Wu Yanjun Xu Changjie and Zhang Shanglong |
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| Institution: | Wu Yanjun 1,2,Xu Changjie 1,and Zhang Shanglong 1 |
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| Abstract: | Tissue culture and genetic transformation are two very important ways which are being used in plant breed ing.Re -cent advances in tis sue culture and genetic transformation of peach are reviewed.This review fo cuses on two methods of tis-sue culture:meristem and shoot-tip culture,embryo culture and two methods of genetic transformation:Agrobacterinm tume -faciens,particle bombardment ,which have been widely used in peach breeding.Various factors affecting in vitro estab lish-ment and growth of peach shoots,such as variety,explants type and stage of develop ment ,culture medium,growth reg ula-tors and culture conditions are illustrated.The key problems ,which affect genetic transformation of peach,are primary ana-lyzed,and focal points of future research are suggested as well. |
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| Keywords: | Peach Tissue culture Genetic transformation |
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