| 用玉米芯酸酶法制备低聚木糖的研究 |
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| 引用本文： | 杨书艳,徐春梅,邬敏辰.用玉米芯酸酶法制备低聚木糖的研究[J].安徽农业科学,2007,35(12):3651-3653. |
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| 作者姓名： | 杨书艳 徐春梅 邬敏辰 |
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| 作者单位： | 1. 江南大学食品学院,江苏无锡,214036;2. 江南大学食品学院,江苏无锡,214036;江南大学医学系,江苏无锡,214064 |
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| 摘 要： | 研究了采用酸酶法水解玉米芯中的木聚糖制备低聚木糖的工艺条件.玉米芯预处理工艺:用60 ℃水浸泡玉米芯12 h,过滤,保留滤渣.玉米芯酸预水解条件:按固液比1∶6加入2.0 g/L的稀硫酸液,120 ℃预水解60 min,溶出总糖量15.01%,平均聚合度2.16.酶水解条件:pH值4.8,加酶量40 IU/g玉米芯,50 ℃水解4 h,溶出总糖量20.32%,平均聚合度1.74,玉米芯酸酶水解液中低聚木糖的相对含量达到62.37%.
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| 关 键 词： | 玉米芯 酸酶法 木聚糖酶 低聚木糖 |
| 文章编号： | 0517-6611（2007）12-03651-03 |
| 收稿时间： | 2006-12-13 |
| 修稿时间： | 2006-12-13 |
Study on the Preparation of Xylooligosacchrides from Corncob with Acid-xylanase Hydrolysis |
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| YANG Shu-yan, et al.Study on the Preparation of Xylooligosacchrides from Corncob with Acid-xylanase Hydrolysis[J].Journal of Anhui Agricultural Sciences,2007,35(12):3651-3653. |
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| Authors: | YANG Shu-yan |
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| Institution: | School of Food Science,Scuthem Yangtze University,Wuxi,Jiangsu 214036 |
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| Abstract: | The preparation process of xylooligosacchrides from corncob with acid-xylanase hydrolysis was systematically studied.The primary treatment method was that the corncob was soaked in water at 60 ℃ for 12 hours,and then filtrated and dryed.The acidic primary hydrolysis was steamed at 120 ℃ for 1 hour(10 g corncob in 60 ml of 2.0 g/L H2SO4).The yield of total sugar was 15.01% and DP 2.16.The conditions of enzymatic hydrolysis were pH 4.8,the amount of xylanase 40 IU/g corncob,and at 50 ℃ for 4 hours.The yield of total sugar was 20.32% and DP 1.74.The purity of xylooligosacchrides was 62.37%. |
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| Keywords: | Corncob Acid-xylanase hydrolysis Xylanase Xylooligosacchrides |
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