| 牛病毒性腹泻—黏膜病病毒C24V株E0截短基因密码子优化及其在卡介苗中的表达 |
| |
| 引用本文： | 张云,郝俊伟,刘红娜,等.牛病毒性腹泻—黏膜病病毒C24V株E0截短基因密码子优化及其在卡介苗中的表达[J].中国畜牧兽医,2014,41(3):71-76. |
| |
| 作者姓名： | 张云 郝俊伟 刘红娜 等 |
| |
| 摘 要： |
|
| 收稿时间： | 2013-10-08 |
Codon Optimization and Expression in BCG of E0 Truncated Gene from C24V Strain of Bovine Viral Diarrhea-Mucosal Disease Virus |
| |
| ZHANG Yun,HAO Jun-wei,LIU Hong-na,WANG Wen-yu,YANG Yu-hang,SHI Kun,LI Jian-ming,DU Rui.Codon Optimization and Expression in BCG of E0 Truncated Gene from C24V Strain of Bovine Viral Diarrhea-Mucosal Disease Virus[J].China Animal Husbandry & Veterinary Medicine,2014,41(3):71-76. |
| |
| Authors: | ZHANG Yun HAO Jun-wei LIU Hong-na WANG Wen-yu YANG Yu-hang SHI Kun LI Jian-ming DU Rui |
| |
| Institution: | (1. College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China;2. College of Chinese Medicinal Material, Jilin Agricultural University, Changchun 130118, China) |
| |
| Abstract: | The protein encoded by E0 gene is one of the major protective antigens of bovine viral diarrhea-mucosal disease virus (BVDV). E0 gene is a candidate for developing genetic engineering vaccine of BVDV and it could not express in BCG, it has been shown that E0 protein has RNase activity, and has a plurality of rare codons according to analyze the codon usage frequency of BCG. By truncating the gene and optimizing its codons, E0 gene expressed in BCG successfully and its antigenic activity was detected, which laid the foundation for building bivalent genetic engineering vaccine to prevent tuberculosis and bovine viral diarrhea-mucosal disease at the same time. |
| |
| Keywords: | bovine viral diarrhea-mucosal disease virus E0 gene codon recombinant BCG |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《中国畜牧兽医》浏览原始摘要信息 |
| 点击此处可从《中国畜牧兽医》下载免费的PDF全文 |
|
