酶法水相提取大豆油难点的解决方法

大豆含油量较低，酶法水相提油困难。为提高得率而加大酶用量，则成本高，无法应用。本研究以水浸提大豆含油蛋白，最适条件下96％大豆油随蛋白进入水提液，少量蛋白酶降解水液中的大豆蛋白，使部分大豆油释放，再离心分离，释放的部分大豆油被分离出的蛋白吸附，得到高作含油量的大豆蛋白则可按已有的适用于高含油量油料的水提法进行提油，解决了大豆油酶法水提的难点。

The Method for Overcoming the Difficulty in Enzymatic Aqueous Extraction Soybean Oil

Owing to the low oil content of soybean, enzymatic aqueous extraction of soybean oil is difficult. In order to increase yield, large amount of enzyme is added, thus the cost is too high by using the technology of oil extraction. In this paper, the oil containing protein soybean is extracted by water, 96% of soybean oil in aqueous solution with protein at optimum technological conditions. The soybean protein in the aqueous solution is hydrolyzed by using a little protease, then a part of soybean oil is released. The high oil content protein which is absorbed by released oil is separated by centrifugation, the oil content of separated protein is 40. 5%, the total oil yield reached 93. 4% by optimizing separation technology. The high oil-containing soybean protein is extracted according to original aqueous extraction method which is suited for high oil content oilseed, the difficulty of enzymatic aqueous extraction soybean oil can be solved.