| HPLC法测定猪血浆中金霉素的残留量* |
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| 引用本文： | 杨秀娟,刘勇,钱朝海,葛长荣,张曦.HPLC法测定猪血浆中金霉素的残留量*[J].云南农业大学学报,2006,21(6):791-793. |
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| 作者姓名： | 杨秀娟 刘勇 钱朝海 葛长荣 张曦 |
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| 作者单位： | (1.云南农业大学,云南省动物营养与饲料重点实验室,云南 昆明 650201;;2.云南省饲料监察所,云南 昆明 650021) |
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| 摘 要： | 采用高效液相色谱法(HPLC)测定猪血浆中的CTC残留量。试验采用Agilent HP1100系统,Agilent ODS C18(150mm×4.6×5)柱,流动相选用乙腈—0.01mol/L草酸(3+2);检测波长为372nm;进样量:50μL;柱温:室温。血浆样品经草酸提取后,过C18柱净化,该方法样品前处理简单,药物的线性关系好,相关系数达0.99989。检测限为0.05μg/mL,平均回收率在75%以上,变异系数均<6.6%。该法前处理简便、灵敏度高、结果准确、可靠。
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| 关 键 词： | 猪血浆 金霉素 高效液相色谱 |
| 收稿时间： | 2006-7-4 |
The Detemination of Chlortetracycline Residues in Pig Plasma by HPLC |
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| YANG Xiu-juan,LIU Yong,QIAN Chao-hai,GE Chang-rong,ZHANG Xi.The Detemination of Chlortetracycline Residues in Pig Plasma by HPLC[J].Journal of Yunnan Agricultural University,2006,21(6):791-793. |
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| Authors: | YANG Xiu-juan LIU Yong QIAN Chao-hai GE Chang-rong ZHANG Xi |
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| Institution: | (1.Yunnan Animal Nutrition and Feed Key Lab,Y A U,Kunming 650201,China;2.Yunnan Feed Minitor Institudtion,Kunming 650021,China) |
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| Abstract: | This experiment uses (HPLC) to determine the chlortetracycline residues amount in the pig plasma. The experiment uses the Agilent HP1100 system, the Agilent ODS C18 (150mm×4.6×5) column, mobile phrase selects acetonitrile-0.017mol/L oxalic acid (3+2); The examination wave length is 372nm, enters the type quantity: 50μL, Column warm: Room temperature. The plama is extracted by oxalic acid,then purify by C18. Sample pretreatment of this method is simply, the correlation coefficient can reache above 0.99989. The examination limits is 0.05μg/g, average recovery above 75%, coefficient of variation is below 6.6%. Sample pretreatment of this method is easily, the result is reliable, and with high sensitivity. |
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| Keywords: | pig plasma Chlortetracycline high liquid chromatography |
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