IBDV强毒株感染SPF鸡后法氏囊淋巴细胞的凋亡及调控机制

为探究IBDV强毒BC6 / 85株感染SPF鸡后 1～ 9d内法氏囊淋巴细胞中Bcl 2和Bax蛋白的动态表达与淋巴细胞凋亡的关系 ,通过透射电镜观察及TUNEL法检测凋亡的淋巴细胞 ,用免疫组织化学方法检测Bcl 2和Bax蛋白。研究结果表明 :感染后 3～ 5d可见到大量凋亡的淋巴细胞 ,Bcl 2和Bax蛋白表达量显著高于同时点的对照组 (P <0 0 1) ;Bcl 2 /Bax(Bcl 2与Bax比率 )显著低于同时点的对照组 (P <0 0 1)。BC6 / 85株感染SPF雏鸡3～ 5d后法氏囊淋巴细胞会发生大量凋亡 ,Bcl 2 /Bax能准确反映淋巴细胞的凋亡程度。

Apoptosis and dynamic expression of Bax and Bcl-2 in lymphocytes of bursa of SPF chickens infected with BC6/85

The objective of the present study was to investigate the apoptosis and dynamic expression of Bcl 2 or Bax in lymphocytes of bursa of 21 day old SPF chickens infected with BC6/85 from the first to ninth day post infection(PI). The apoptosis was observed by electron microscope, the apoptotic rate was assessed by TUNEL method, and the expressions of Bcl 2, Bax were investigated by immunohistochemical ABC method. The results showed that the apoptosis of lymphocytes in bursa of chickens infected with BC6/85 from the third to fifth day PI significantly increased than that of normal SPF chickens( P <0 01). The expression of Bcl 2 or Bax was significantly increased in lymphocytes of bursa of SPF chickens infected with BC6/85 from the third to fifth day PI than in those of normal chickens( P <0 01). The ratio of Bcl 2 to Bax was significantly decreased compared with that of normal chickens from the third to fifth day PI( P <0 01). The results demonstrate that a great deal of lymphocyte disappearance in the bursa of SPF chickens infected with BC6/85 from the third to fifth day is related to apoptosis. The ratio Bcl 2 to Bax could precisely reflect the degree of lymphocyte apoptosis of bursa.