抗甜菜褐斑病体细胞无性系变异的研究

本试验将甜菜褐斑病菌毒素作为选择压力，对毒素毒性活力进行生物学测定后，设置不同的浓度梯度，诱导未授粉胚珠的抗性愈伤组织，进而诱导再生植株。对再生植株进行抗病性鉴定，采用分生孢子定量接种、抑制胚根生长及田间试验，确定经过毒素处理的再生植株对甜菜褐斑病的抗性水平；对甜菜褐斑病病原菌毒素粗提液的合适使用量进行了初探，对经该病原菌培养滤液处理后得到的再生植株，采用一步或分步淘汰法，提高了再生植株的抗病水平。确定了利用甜菜褐斑病菌毒素作为选择压力的抗甜菜褐斑病体细胞无性系变异的操作流程，即利用未授粉胚珠培养诱导抗性愈伤组织，在选择培养基上诱导再生植株，经筛选淘汰后，壮苗并生根，进行移栽加倍，获得纯合株系，经抗病性鉴定后，从中选育抗病品系。

Study on somaclonal variation of sugarbeet leafspot resistance

In this work，we used toxic of Cercospora biotia as the selecting pressure，after measuring the activity of the toxicto induce calli of the unpollinated ovule of sugarbeet，then obtain the regeneration plants.We identified the resistance to Cercospora leafspot using conidispore inoculation，innibited the growth of embryo root and field test，to evaluate the resistant levels of the regeneration plants to Cercospora leafspot.We study the suitable dose of the toxicin the meantimeusing one or with the methods to supersede unvariated regeneration plants step by step.We created the procedure of the somaclonal variationfirst we induced calli in selecting medium，then induced regeneration plants，after superseded，replanted the regenration plants，doubled the chromosomes，obtained the diploid.